Nitrogen Fixation Explained: How to Choose Cover Crops That Feed Your Soil
On this page ()
What Is Biological Nitrogen Fixation?
Nitrogen is the nutrient most commonly limiting plant growth in tropical plantation soils. While synthetic fertilizers can supply nitrogen, they are expensive, subject to price volatility, and contribute to soil acidification over time. Biological nitrogen fixation adds nitrogen from the air.
Leguminous cover crops form a symbiotic relationship with soil bacteria called rhizobia (for these tropical legumes, mainly Bradyrhizobium). These bacteria colonize the roots of legume plants, forming visible nodules where they convert atmospheric nitrogen gas (N2) into ammonia (NH3), a form that plants can absorb and use. In return, the plant provides the bacteria with carbohydrates produced through photosynthesis.
This process is entirely natural and self-sustaining. Legume cover contributes nitrogen; adjust your fertiliser programme only after soil and leaf analysis shows what the cover supplies on your block.
What Is Measured for Each Species
Not all cover crop species fix nitrogen at the same rate. The amount of nitrogen fixed depends on the species, soil conditions, climate, and management practices. Here is what has been measured for the four main tropical cover crop species available through Kudzu Seeds Trading (see our full 5-species comparison for additional detail).
Mucuna bracteata (MB): 67 to 84% of its nitrogen from the air
Mucuna bracteata is a vigorous, high-biomass cover crop with extensive root systems, and it draws most of its nitrogen from the air: 67 to 84% in a 15N isotope-dilution study under three-year-old oil palm on Jawa Series soil in Malaysia (Cheah, Zaharah and Aminuddin 2010). That is a share, not a quantity; no routine annual fixation rate is asserted for MB.
However, how fast MB closes depends on the site: about 100% ground cover at 6 months with 680 seedlings per hectare on mineral soil under oil palm (Samedani et al. 2015), but 31% at 6 months and 78% at 24 months with 320 seedlings per hectare on deep peat (MPOB TT No. 501), so on slower sites the nitrogen benefit builds gradually. Because no annual rate is asserted for MB, adjust your fertiliser programme only after soil and leaf analysis shows what the cover supplies on your block.
Pueraria javanica (PJ): up to 250 kg N/ha/year accumulated in one trial in Thailand
Pueraria javanica offers a strong balance between establishment speed and nitrogen fixation. It covers steadily (PROSEA 11 records about 60 to 70% cover at about 4 months and 90 to 100% at 8 months) and keeps contributing nitrogen while the stand persists. PJ is a reliable all-round performer.
PJ is well-suited to situations where steady establishment and substantial nitrogen fixation are needed. It tolerates moderate shade, but expect less growth and nitrogen as tree crop canopies close. The one sourced figure, up to 250 kg N/ha per year accumulated in above-ground biomass, comes from a single trial under young rubber in northeast Thailand (Clermont-Dauphin et al. 2016).
Calopogonium mucunoides (CM): no verified annual rate
We do not quote a nitrogen figure for Calopogonium mucunoides, because the published figures come from single short trials; its strength is rapid establishment. It covers the soil 3 to 6 months after sowing, sooner on newly cleared fertile land, with a dense sward at 4 to 5 months (PROSEA 11), making it valuable where early cover and an early nitrogen contribution matter.
CM is often used as a pioneer species in mixed plantings. While it may be outcompeted by more vigorous species over time, its early nitrogen contribution helps establish soil fertility during the critical first year of a plantation development or replanting cycle.
Centrosema pubescens (CP): no verified annual rate
We do not quote a nitrogen figure for Centrosema pubescens, because the published estimates are wide and not tied to plantation trials; it distinguishes itself through shade tolerance. In mature plantations where canopy closure limits light availability, CP continues to fix nitrogen when other species decline. This makes it particularly valuable in coconut and older oil palm plantations.
CP's persistence under shade means it provides a reliable, long-term nitrogen source in conditions where other cover crops struggle to maintain productivity.

Factors That Affect Nitrogen Fixation Efficiency
Soil pH is a critical factor. Most rhizobia perform best in moderately acid to neutral soils, and strongly acidic soils can inhibit nodulation and reduce nitrogen fixation efficiency. If your soil is strongly acidic, consider liming before establishing cover crops.
Soil phosphorus availability also matters. Rhizobia require phosphorus for energy metabolism, and nitrogen fixation is an energy-intensive process. Soils deficient in phosphorus may limit the nitrogen fixation potential of cover crops. A soil test before planting can identify this issue.
Drainage is important because waterlogged soils reduce oxygen availability to root nodules, impairing nitrogen fixation. Well-drained soils support more efficient fixation. On poorly drained sites, consider raised planting beds or drainage improvements.
Maximizing Nitrogen Return to Your Soil
To maximize the nitrogen benefit from cover crops, consider these management practices. Allow cover crops to grow for at least one full season before any cutting or slashing. This gives the plants time to build root systems and accumulate fixed nitrogen in their biomass.
When slashing is necessary for access or management, leave the cut material on the soil surface as mulch. As this material decomposes, the nitrogen it contains is released into the soil and becomes available to tree crops.
Avoid applying synthetic nitrogen fertilizer directly to cover crop areas. Excess available nitrogen in the soil can actually suppress biological fixation, as the rhizobia reduce activity when nitrogen is already abundant.
Growers also use SoilBoost EA humic acid soil conditioner alongside cover crops as part of a broader programme. It may support soil structure and root-zone conditions during the establishment phase; it does not supply rhizobia and is not shown to increase nodulation or nitrogen fixation.
Making Your Decision
The right cover crop depends on your specific priorities. If dense, long-lasting ground cover is your goal and you can wait for establishment, consider Mucuna bracteata. If you need a balance of establishment speed and ground cover, consider Pueraria javanica. For shaded conditions, consider Centrosema pubescens, which keeps growing as canopies close. And for rapid early coverage, Calopogonium mucunoides gets started fast.
Many plantation managers achieve the best results with mixed-species plantings that combine two or three species to cover different niches and provide resilience against changing conditions.
Request a quote or contact Kudzu Seeds Trading on WhatsApp at +60 17-237 4058 to discuss which species or combination best fits your plantation conditions, or browse our full seed catalog.